Label-free plasmonic-based biosensing using a gold nanohole array chip coated with a wafer-scale deposited WS2 monolayer

Lixing Kang; Yan Zhang; Qian Gong; Chandreyee Manas Das; Huilin Shao; Daniel Puiu Poenar; Philippe Coquet; Ken-Tye Yong

doi:10.1039/D2RA03479D

Label-free plasmonic-based biosensing using a gold nanohole array chip coated with a wafer-scale deposited WS₂ monolayer†

Lixing Kang,

‡*^abc Yan Zhang,‡^de Qian Gong,‡^c Chandreyee Manas Das,^ab Huilin Shao,^de Daniel Puiu Poenar,*^ab Philippe Coquet^af and Ken-Tye Yong

Author affiliations

* Corresponding authors

^a CINTRA CNRS/NTU/THALES, UMI 3288, Research Techno Plaza, 50 Nanyang Drive, Border X Block, Singapore 637553, Singapore
E-mail: lxkang2013@sinano.ac.cn

^b School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
E-mail: EPDPuiu@ntu.edu.sg

^c Division of Advanced Materials, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

^d Institute for Health Innovation & Technology, National University of Singapore, Singapore 117583, Singapore

^e Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117583, Singapore

^f Institut d’Electronique, de Microélectronique et de Nanotechnologie (IEMN), CNRS UMR 8520 – Université de Lille 1, Villeneuve d’Ascq 59650, France

^g The University of Sydney Nano Institute, The University of Sydney, Sydney 2006, New South Wales, Australia

Abstract

This paper reports the fabrication, testing and obtained performance of a plasmonic sensor employing a gold (Au) nanohole array chip coated with tungsten disulphide (WS₂), which is then functionalized for the detection of protein–protein interactions. A key novelty is that the WS₂ was deposited as a monoatomic layer using a wafer-scale synthesis method that successfully provided a film of both high quality and uniform thickness. The deposited WS₂ film was transferred onto a Au nanohole array chip using a novel method and was subsequently functionalized with biotin. The final sensor was tested and it demonstrated efficient real-time and label-free plasmonic detection of biotin–streptavidin coupling. Specifically, compared to a standard (i.e. uncoated) Au nanohole-based sensor, our WS₂-coated Au nanohole array boosted the spectral shift of the resonance wavelength by ∼190%, resulting in a 7.64-fold improvement of the limit of detection (LOD).

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Article information

DOI: https://doi.org/10.1039/D2RA03479D
Article type: Paper
Submitted: 05 Jun 2022
Accepted: 07 Nov 2022
First published: 21 Nov 2022
This article is Open Access

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RSC Adv., 2022,12, 33284-33292

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Label-free plasmonic-based biosensing using a gold nanohole array chip coated with a wafer-scale deposited WS₂ monolayer

L. Kang, Y. Zhang, Q. Gong, C. M. Das, H. Shao, D. P. Poenar, P. Coquet and K. Yong, RSC Adv., 2022, 12, 33284 DOI: 10.1039/D2RA03479D

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