Structural insights into base excision repair
As the instruction manual of the cell, DNA plays an essential role in development and organism function. Consequently, several mechanisms exist to repair DNA following damage. Oxidants result in structural damage to DNA which are typically repaired via base excision repair (BER). A set of proteins called glycosylases serve to recognize these DNA lesions and initiate BER. hNEIL1, a human glycosylase, has been linked to a wide variety of DNA lesions but primarily studied in the context of thymine glycol (Tg) lesions. Furthermore, hNEIL1 has two isoforms, edited and unedited, which act differentially on Tg lesions.
Rachana Tomar from the Stone lab, alongside collaborators in the Iverson and Egli labs, recently published a paper in Nucleic Acids Research that investigated both forms of hNEIL1 within the context of urea lesions. Using a combination of biochemical and structural techniques, they were able to determine the structures and activity of both glycosylase isoforms when bound to urea DNA lesions. While the unedited form of hNEIL1 exhibited a novel intermediate pre-cleavage state, there was no significant difference in efficiency of urea lesion removal between the two isoforms.
This study sheds light on the mechanism of hNEIL1 function between different DNA lesions and provides a basis for further research in base excision repair pathways.
Read the full article here! ~C.I.C.
Frontiers in Biochemistry Seminar Series Departmental Awards
Alex Blee, Chazin Lab (Michael Waterman Outstanding Achievement in Postdoctoral Studies Award)
Predicting XPA variants of unknown significance that impact chemotherapy response
TODAY, 12pm, 214 LH
Add this to your calendar
CryoEM Discussion Group
Ruojing Zhang, Mchaourab Lab
From Novice to Structure Builder: A Journey in CryoEM
May 9, 2pm, 5131 MRB3 or Zoom
Meeting ID: 967 7521 3705
Passcode: 611949
Add this to your calendar
Other Seminars & Events
ASPIRE Cafe for Postdoctoral Fellows
Neil Dani, Assistant Professor, CDB
Kathy DelGiorno, Assistant Professor, CDB
Setting up and Running a New Lab
May 11, 11am, 214 LH
More information
Dissertation Defense Presentation
Ivette Perez, Iverson Lab
Arrestin-mediated Effector Activation
May 16, 10am, 208 LH
Add this to your calendar
Pharmacology Seminar Series
Tracy M. Handel, UC San Diego
Atypical Structure and Function of Typical Chemokine Receptors
May 16, 4pm, 214 LH
Add this to your calendar
2023 Careers in Pharmaceutical Industry Discovery Day
Co-hosted by Molecular Biophysics Training Program
Speakers from AstraZeneca, Merck and Pfizer
May 17, 1pm-4pm, 208 LH
Register here
2023 Mid-South Biophysics Symposium
A Biophysical Society Networking Event
Keynote speaker: Marija Zanic, Vanderbilt University
May 22, University of Mississippi, Oxford, MS
Abstract Priority deadline: April 21, 2023
Website and Abstract Portal
VI4 Seminar Series
David Savage, UC Berkeley
May 23, 10:30am, 512 LH
Co-sponsored by the CSB
Save the Date
ASPIRE Annual Career Symposium
A Kaleidoscope of Biomedical Science Careers
June 2, Student Life Center
More information and Register here
ASPIRE Job Search Series for PhD Students and Postdocs
Kate Stuart, BRET Office of Career Development
Cover Letters
June 8, 11am, 214 LH
More information
The Protein Society Annual Symposium
PS37 Boston
July 13 – 16
Register here
|