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Golgi

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Yanzhuang Wang

    1. Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Vladimir V Lupashin

    1. Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Todd R. Graham

    1. Department of Biological Sciences, Vanderbilt University, Nashville, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2557)

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Table of contents (48 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Model Systems Used to Study Golgi Organization and Function

    1. Front Matter

      Pages 1-1
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    2. Live Cell Imaging of Yeast Golgi Dynamics

      • Giancarlo Costaguta, Gregory S. Payne, Lydia Daboussi
      Pages 3-15

    3. Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuole

      • Mariana Jimenez, Jordan T. Best, Swapneeta S. Date, Todd R. Graham
      Pages 17-28

    4. Immunofluorescence as a Method to Study Golgi Organization in Larval Salivary Glands of Drosophila melanogaster

      • Tetyana Chorna, Julie A. Brill
      Pages 29-37

    5. Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golgi

      • Sang-Jin Kim, Federica Brandizzi
      Pages 39-51

    6. Reconstitution of Golgi Biogenesis in Permeabilized Trypanosoma brucei Cells

      • Graham Warren
      Pages 53-60

    7. Generation of GM130 Conditional Knockout Mouse

      • Mei Mei, Shilai Bao
      Pages 61-81

    8. Monitoring Effects of Membrane Traffic Via Changes in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts

      • Maha M. Hamed, Kenichiro Taniguchi, Mara C. Duncan
      Pages 83-98

  3. Morphological Studies of Golgi Organization and Function

    1. Front Matter

      Pages 99-99
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    2. Immunofluorescence Microscopy of the Mammalian Golgi Apparatus

      • Maryam Arab, Sanjeev Chavan Nayak, Teresa Vitali, Martin Lowe
      Pages 101-111

    3. Studying the Organization of the Golgi by Super-Resolution Microscopy

      • Hieng Chiong Tie, Lei Lu
      Pages 113-125

    4. Super-Resolution Live Imaging of Cargo Traffic Through the Golgi Apparatus in Mammalian Cells

      • Takuro Tojima, Daisuke Miyashiro, Yasuhito Kosugi, Akihiko Nakano
      Pages 127-140

    5. Studying Golgi Structure and Function by Thin Section TEM

      • Helena Vihinen, Eija Jokitalo
      Pages 141-159

    6. Algorithm for Modern Electron Microscopic Examination of the Golgi Complex

      • Alexander A. Mironov, Galina V. Beznoussenko
      Pages 161-209

    7. High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking

      • Shijie Liu, Irina D. Pokrovskaya, Brian Storrie
      Pages 211-223

    8. Negative Staining Electron Microscopy for Morpho-Functional Characterization of Purified Golgi Membranes from Mammalian Cells

      • Stefano De Tito, Gabriele Turacchio, Carmen Valente
      Pages 225-234

    9. An Electron Tomographic Analysis of Giantin-Deficient Golgi Proposes a New Function of the Golgin Protein Family

      • Ayano Satoh, Mitsuko Hayashi-Nishino, Kunihiko Nishino
      Pages 235-246

    10. Modeling the Cryo-EM Structure of the Exocyst Complex

      • Kunrong Mei, Wei Guo
      Pages 247-262

    11. Quantitatively Assessing Co-Localization of Golgi Proteins by Distance Analysis Using the DiAna Software

      • Nina Freimuth, Michael Sauer
      Pages 263-274
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Keywords

About this book

This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study Golgi formation, maintenance, and functions under physiological and pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Golgi: Methods and Protocols is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function.


Editors and Affiliations

  • Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, USA

    Yanzhuang Wang

  • Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, USA

    Vladimir V Lupashin

  • Department of Biological Sciences, Vanderbilt University, Nashville, USA

    Todd R. Graham

Bibliographic Information

  • Book Title: Golgi

  • Book Subtitle: Methods and Protocols

  • Editors: Yanzhuang Wang, Vladimir V Lupashin, Todd R. Graham

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2639-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2638-2Published: 14 December 2022

  • Softcover ISBN: 978-1-0716-2641-2Published: 14 December 2023

  • eBook ISBN: 978-1-0716-2639-9Published: 13 December 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 816

  • Number of Illustrations: 29 b/w illustrations, 167 illustrations in colour

  • Topics: Cell Biology

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