Comparison against current spectral flow cytometers (e.g., Cytek Aurora, Sony ID7000) offer high-parameter capabilities but often suffer from:
- Fixed laser/detector configurations, limiting long-term scalability
- Opaque and inflexible spectral unmixing algorithms, complicating panel optimization
Suboptimal performance at low signal-to-noise ratios, particularly for dim markers and rare populations
- Complex, error-prone compensation workflows, and steep learning curves for non-expert users
- Throughput limitations, especially in large-scale clinical or high-throughput screening applications
These constraints create bottlenecks in translational studies that demand precision, reproducibility, and adaptability over time.
Advantages of NovoCyte Opteon:
- Modular Design for Future-Proofing
Configurable with up to 5 lasers and 73 detection channels
Lasers and detectors are field-upgradable, enabling iterative expansion as experimental needs grow—without hardware replacement or extended downtime
- High-Resolution Spectral Detection
Optimized optics and spectral detection enable accurate discrimination of fluorochromes with high spectral overlap
Facilitates design of >30-color panels with reduced need for custom antibody-fluor combinations
- Advanced Spectral Unmixing and Analysis Tools
Real-time unmixing with visual diagnostic plots improves transparency and interpretability
Enhanced autofluorescence subtraction improves resolution in tissue-based and clinical samples
Intuitive software reduces training time and minimizes technical errors during panel setup
- Superior Fluidics and Throughput
Incorporates Agilent’s high-precision fluidics for stable flow rates and reduced clogging
Supports high-throughput sample acquisition with integrated plate loaders and autosampler modules—critical for longitudinal or cohort studies
- Preservation of Rare and Dim Populations
High sensitivity enables robust detection of low-abundance markers (e.g., transcription factors, cytokines)
Preserves viability and integrity of rare subsets, e.g., tumor-infiltrating lymphocytes, exhausted T cells, and circulating stem cells
- Seamless Data Export and Integration
Compatible with FlowJo, Cytobank, and other single-cell analysis pipelines
Data structure and metadata are FAIR-compliant, facilitating cross-study integration and machine learning-based downstream analysis
Conclusion: The NovoCyte Opteon provides a next-generation spectral cytometry solution with unmatched flexibility, throughput, and analytical power.
Next steps: Informational demos were conducted during the Grand Opening (April 2025) and additional training sessions can be scheduled for new users. For inquiries or to discuss your high‑dimensional flow needs, please contact the Center (k3perry@health.ucsd.edu).
