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Measure across scales

Quantitative biology is a frontier at the intersection of mathematics, statistics, and computer science. By constructing predictive models grounded in the fundamental principles of living systems, quantitative biology unveils profound secrets that are pivotal to our understanding of existence. Imagine delving into questions that challenge the very essence of what it means to be alive: How do the microscopic cells within our bodies exhibit such remarkable intelligence and adaptability under unpredictable conditions? What intricate architectures and emergent properties within these cellular networks empower them to seamlessly learn and evolve? The answers lie within the reach of quantitative biology, offering the potential to transform our comprehension of life’s complexity and drive groundbreaking advancements across science and medicine.

The Agilent CoE is packed with high-end equipment that serves as a shared resource, allowing for diverse research in quantitative biology. The 
instruments grant the ability to ask and answer questions from bioenergetics to cellular impedance, and from multi-omics to immunophenotyping, and everything in between. We invite the campus, local biotech, CROs and sister institutions (SALK, Scripps and LJI) to benefit from these valuable resources. Listed below are the various instruments and their distinguishing features. 

The BioTek Cytation C10 confocal imaging reader combines automated confocal and widefield microscopy with conventional multimode microplate reading. The patented confocal design gives exquisite resolution and optical sectioning capabilities for many sample types. A Hamamatsu scientific CMOS camera, Olympus objectives, and laser-based illumination deliver high-quality images. Cytation C10 also includes widefield fluorescence, brightfield and phase contrast optics. The variable bandwidth monochromator-based multimode plate reading is based on the proven design of the Agilent BioTek Synergy products. Environmental controls, 3D viewer, and Gen5 software enable high-quality results for many applications.

Detect and quantify micro- and macro- lipid droplets

Quantify plaques after therapy against Atherosclerosis

Measure ROS in neutrophils in the presence of microbes

Image and quantify differentiated cancer organoids post-therapy

The NovoCyte Penteon flow cytometer provides an expanded set of capabilities that accommodate today's high-end and increasingly sophisticated multi-color flow cytometry assays. You now have the flexibility to choose from up to 30 fluorescence channels utilizing up to 5 lasers with up to 30 independent detectors. The NovoSampler Q can be integrated into different laboratory automation platforms and efficiently processes both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. The intuitive and industry leading NovoExpress software has been further advanced, providing an exceptional user experience in data acquisition, analysis and reporting. 

Immunophenotyping of human monocyte-derived macrophages

The Seahorse XFPro has the ability to detect discrete changes in cellular bioenergetics (all mitochondrial readouts) in real-time which provides a uniquely clear window into the critical functions driving cell signaling, proliferation, activation, toxicity, and biosynthesis.

Bioenergetics of Intestinal Myofibroblasts in Stricturing Crohn's Disease

Cells constantly undergo changes in cell state (division, detachment, adhesion, migration). This instrument can help you keep your hand on the pulse of all of this and more and assess their health by combining Imaging (morphology) & Impedance (adhesion). This enables comprehensive insight into cell health, behavior, and function (e.g., strength of adhesion, changes in morphology, proliferation, and cytolysis) and cell biology processes using live, simultaneous, and real time biosensor impedance-based and image-based measurements.  

The new 6495D triple quadrupole LC/MS system is an ultrahigh-performance system built for research and testing labs investigating large batches of samples. Equipped with the latest, innovative iFunnel technology, this LC/MS/MS achieves ppq-level detection limits for the most challenging analytes in heavy matrix- for example in ‘omics, food, and environmental analyses- while providing high precision at sub-millisecond dwell times. You can now accomplish 3 powerful goals: (i) Population Analysis of the Plasma Lipidome; (ii) End-to-End Targeted Metabolomics; (iii) Quantitative Proteomics Using Standard or Nanoflow HPLC.

With a streamlined profile and smaller footprint, Autostainer Link 48 allows you to standardize and improve throughput in the processing and staining of histopathology slides while minimizing the impact to valuable laboratory bench space.

High-Throughput Readout for Neutrophilic Infiltrates

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The Agilent Center of Excellence (CoE) in Cellular Intelligence represents a partnership that aligns the strategic visions of Agilent Technologies and the UC San Diego Institute for Network Medicine to enhance and modernize quantitative biology. As embraced by their tagline “Unlocking measurement insights for 75 years", Agilent technologies is known for their leadership in innovating technologies that can quantify with precision. This partnership will enable UC San Diego's scientists to conduct biological measurements across scales using a complete and modern suite of cell analyses platforms that reveal cell fate, state and trait through measurable insights across scales. Through pre- and post-doctoral fellowships, this Center also supports the training and education of future generations of scientists.